Pooling strategy and chromosome painting characterize a living zebroid for the first time

نویسندگان

  • Alessandra Iannuzzi
  • Jorge Pereira
  • Clara Iannuzzi
  • Beiyuan Fu
  • Malcolm Ferguson-Smith
چکیده

We have investigated the complex karyotype of a living zebra-donkey hybrid for the first time using chromosome-specific painting probes produced from flow-sorted chromosomes from a zebra (Equus burchelli) and horse (Equus caballus). As the chromosomes proved difficult to distinguish from one another, a successful new strategy was devised to resolve the difficulty and characterize each chromosome. This was based on selecting five panels of whole chromosome painting probes that could differentiate zebra and donkey chromosomes by labelling the probes with either FITC or Cy3 fluorochromes. Each panel was hybridized sequentially to the same G-Q-banded metaphases and the results combined so that every zebra and donkey chromosome in each suitable metaphase could be identified. A diploid number of 2n = 53, XY was found, containing haploid sets of 22 chromosomes from the zebra and 31 chromosomes from the donkey, without evidence of chromosome rearrangement. This new strategy, developed for the first time, may have several applications in the resolution of other complex hybrid karyotypes and chromosomal aberrations.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Incorporating location, routing, and inventory decisions in a bi-objective supply chain design problem with risk-pooling

This paper considers a single-sourcing network design problem for a three-level supply chain. For the first time, a novel mathematical model is presented considering risk-pooling, the inventory existence at distribution centers (DCs) under demand uncertainty, the existence of several alternatives to transport the product between facilities, and routing of vehicles from distribution centers to c...

متن کامل

The study and recognition of artistic dyes in the Islamic period of Iran in writing and painting (Based on poetry of Khorasanid style poets)

The main features of Iranian painting in the post-Islamic centuries are the association with Persian literature. Persian literature and Persian art have intrinsic links, since the artist and poet are based on the unit's vision, rooted in a culture and intellectual space, to create. The result of this poet's creation is a literary work, and this work can have all the features of the work of art....

متن کامل

Criteria of Artistic Expression in the First and Second Half of Qajar Era with an Emphasis on the Murals of Royal Reception in Negarestan Palace and Nezamiyeh Mansion

Wall painting stands among the prominent art media for displaying royal themes in Qajar era. There were many murals in the palaces and buildings of that period now mostly destroyed, however descriptions or pictures of which are available in historical texts to study and observe. The royal (New Year) reception wall painting of Fath Ali Shah in Negarestan palace and that of Naser ad-Din Shah in N...

متن کامل

Association of Chromosome Territories with the Nuclear Matrix

To study the possible role of the nuclear matrix in chromosome territory organization, normal human fibroblast cells are treated in situ via classic isolation procedures for nuclear matrix in the absence of nuclease (e.g., DNase I) digestion, followed by chromosome painting. We report for the first time that chromosome territories are maintained intact on the nuclear matrix. In contrast, comple...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 12  شماره 

صفحات  -

تاریخ انتشار 2017